Q1 · Blotting Techniques
NEET PGMediumWestern blotting is used to detect?
Explanation
Southern blotting detects DNA, Northern detects RNA and Western detects protein. Western blot has been used, for example, as a confirmatory HIV test.
Free Molecular Biology practice for NEET PG and INI-CET Biochemistry, with a written explanation on every question.
6 questions · Free explanations
Showing 1–6 of 6 questions
Q1 · Blotting Techniques
NEET PGMediumExplanation
Southern blotting detects DNA, Northern detects RNA and Western detects protein. Western blot has been used, for example, as a confirmatory HIV test.
Q2 · Polymerase Chain Reaction
NEET PGEasyExplanation
PCR uses heat-stable Taq DNA polymerase to amplify DNA through repeated cycles of denaturation, annealing and extension. Taq's thermostability allows it to survive the high denaturation temperatures.
Q3 · DNA Replication
HardExplanation
DNA polymerase I uses its 5' to 3' exonuclease activity to remove RNA primers and fills the gaps with DNA. DNA ligase then seals the remaining nicks between Okazaki fragments.
Q4 · Genetic Code
MediumExplanation
The code is degenerate, or redundant, because most amino acids are specified by more than one codon. This buffers the effect of some point mutations, especially at the third wobble position.
Q5 · Transcription
EasyExplanation
Transcription is the synthesis of mRNA from a DNA template by RNA polymerase. Translation then converts the mRNA sequence into protein at the ribosome.
Q6 · Mutations
HardExplanation
A nonsense mutation changes a codon to a premature stop codon, truncating the protein. A missense mutation changes one amino acid, a silent mutation causes no amino-acid change and a frameshift shifts the reading frame.
Molecular Biology sits within Biochemistry, which carries the same syllabus in both exams. This set runs to 6 questions, each tagged Easy, Medium or Hard, 2 of them at Hard, and 2 drawn from previous-year papers.
Answer first, then read the explanation even on the ones you got right — the reasoning is where Molecular Biology marks are actually won, and it is what separates a recalled fact from a question you can solve in a new form. Once the Easy set feels comfortable, filter to Hard to find the gaps Biochemistry examiners keep returning to.
FAQ
Yes. Every question and explanation in this Molecular Biology set is free and open — no login, no form and no payment. The explanation appears the moment you answer.
Molecular Biology sits within Biochemistry, which appears in the same form in both syllabuses. Metabolism, enzymes and molecular biology, distilled to the pathways and deficiency states that repeat in previous year papers.
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